Draft Genome Sequences of Five Enterococcus Species Isolated from the Gut of Patients with Suspected Clostridium difficile Infection

Indexación: Scopus.We present draft genome sequences of five Enterococcus species from patients suspected of Clostridium difficile infection. Genome completeness was confirmed by presence of bacterial orthologs (97%). Gene searches using Hidden-Markov models revealed that the isolates harbor between seven and 11 genes involved in antibiotic resistance to tetracyclines, beta-lactams, and vancomycin.http://genomea.asm.org/content/5/20/e00379-17.ful

Robustness of reserve selection procedures under temporal species turnover

Complementarity-based algorithms for the selection of reserve networks emphasize the need to represent biodiversity features efficiently, but this may not be sufficient to maintain those features in the long term. Here, we use data from the Common Birds Census in Britain as an exemplar data set to determine guidelines for the selection of reserve networks which are more robust to temporal turnover in features. The extinction patterns found over the 1981-1991 interval suggest that two such guidelines are to represent species in the best sites where they occur (higher local abundance) and to give priority to the rarer species. We tested five reserve selection strategies, one which finds the minimum representation set and others which incorporate the first or both guidelines proposed. Strategies were tested in terms of their efficiency (inversely related to the total area selected) and effectiveness (inversely related to the percentage of species lost) using data on eight pairs of ten-year intervals. The minimum set strategy was always the most efficient, but suffered higher species loss than the others, suggesting that there is a trade-off between efficiency and effectiveness. A desirable compromise can be achieved by embedding the concerns about the long-term maintenance of the biodiversity features of interest in the complementarity-based algorithms

Comparative Genomics Analysis of a New Exiguobacterium Strain from Salar de Huasco Reveals a Repertoire of Stress-Related Genes and Arsenic Resistance

Indexación: Web of Science; Scopus.The Atacama Desert hosts diverse ecosystems including salt flats and shallow Andean lakes. Several heavy metals are found in the Atacama Desert, and microorganisms growing in this environment show varying levels of resistance/tolerance to copper, tellurium, and arsenic, among others. Herein, we report the genome sequence and comparative genomic analysis of a new Exiguobacterium strain, sp. SH31, isolated from an altiplanic shallow athalassohaline lake. Exiguobacterium sp. SH31 belongs to the phylogenetic Group II and its closest relative is Exiguobacterium sp. S17, isolated from the Argentinian Altiplano (95% average nucleotide identity). Strain SH31 encodes a wide repertoire of proteins required for cadmium, copper, mercury, tellurium, chromium, and arsenic resistance. Of the 34 Exiguobacterium genomes that were inspected, only isolates SH31 and S17 encode the arsenic efflux pump Acr3. Strain SH31 was able to grow in up to 10 mM arsenite and 100 mM arsenate, indicating that it is arsenic resistant. Further, expression of the ars operon and acr3 was strongly induced in response to both toxics, suggesting that the arsenic efflux pump Acr3 mediates arsenic resistance in Exiguobacterium sp. SH31.http://journal.frontiersin.org/article/10.3389/fmicb.2017.00456/ful

Enhanced Recognition of Vocal Emotions in Individuals With Naturally Good Musical Abilities

Music training is widely assumed to enhance several nonmusical abilities, including speech perception, executive functions, reading, and emotion recognition. This assumption is based primarily on cross-sectional comparisons between musicians and nonmusicians. It remains unclear, however, whether training itself is necessary to explain the musician advantages, or whether factors such as innate predispositions and informal musical experience could produce similar effects. Here, we sought to clarify this issue by examining the association between music training, music perception abilities and vocal emotion recognition. The sample (N = 169) comprised musically trained and untrained listeners who varied widely in their musical skills, as assessed through self-report and performance-based measures. The emotion recognition tasks required listeners to categorize emotions in nonverbal vocalizations (e.g., laughter, crying) and in speech prosody. Music training was associated positively with emotion recognition across tasks, but the effect was small. We also found a positive association between music perception abilities and emotion recognition in the entire sample, even with music training held constant. In fact, untrained participants with good musical abilities were as good as highly trained musicians at recognizing vocal emotions. Moreover, the association between music training and emotion recognition was fully mediated by auditory and music perception skills. Thus, in the absence of formal music training, individuals who were “naturally” musical showed musician-like performance at recognizing vocal emotions. These findings highlight an important role for factors other than music training (e.g., predispositions and informal musical experience) in associations between musical and nonmusical domains. (PsycInfo Database Record (c) 2020 APA, all rights reserved

Image Quality In Partially Erased Denoptix® Storage Phosphor Plates

This study aimed at investigating the effect of the partial erasing of DenOptix® system storage phosphor plates on the image quality of digital radiographs. Standardized digital radiographs were acquired of a phantom mandible, using size 2 intraoral DenOptix® storage phosphor plates (n = 10). Subsequently, the active areas of the plates were placed in a viewing box with a constant light intensity of 1,700 lux for 130 seconds to achieve complete erasing (control plate), as well as for 0, 5, 10, 1S, 20, 25, 34, 66, and 98 seconds, to compose the experimental group of partially erased plates. The same exposure settings were repeated using the control and experimental plates, which were scanned at a resolution of 300 dpi. Five radiologists independently examined the pairs of digital radiographs obtained with the control and partially erased plates, in random order, and indicated the best image for oral diagnosis. Cochran-Mantel-Haenszel's chi-square test, at a significance level of 5%, was used to compare the percentages of superior quality images in each combination of control and partially erased plates, subjectively assessed. No significant differences were found between radiographic images acquired with control and partially erased plates, except for the combination of 0 second (30%) versus 130 seconds (70%), p = 0.0047. It can be concluded that, under adequate light intensity conditions, erasing intraoral DenOptix® storage phosphor plates may require time intervals of as little as 5 seconds.2217883Akdeniz, B.G., Gröndahl, H.G., Kose, T., Effect of delayed scanning of storage phosphor plates (2005) Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 99 (5), pp. 603-607Akdeniz, B.G., Gröndahl, H.G., Degradation of storage phosphor images due to scanning delay (2006) Dentomaxillofac Radiol, 35 (2), pp. 74-77Almeida, S.M., Oliveira, A.E.F., Ferreira, R.I., Bóscolo, F.N., Image quality in digital radiographic systems (2003) Braz Dent J, 14 (2), pp. 136-141Borg, E., Attaelmanan, A., Gröndahl, H.G., Subjective image quality of solid-state and photostimulable phosphor systems for digital intra-oral radiography (2000) Dentomaxillofac Radiol, 29 (2), pp. 70-75Ferreira, R.I., Haiter-Neto, F., Tabchoury, C.P.M., Paiva, G.A.N., Bóscolo, F.N., Assessment of enamel demineralization using conventional, digital, and digitized radiography (2006) Braz Oral Res, 20 (2), pp. 114-119Wenzel, A., Gröndahl, H.G., Direct digital radiography in the dental office (1995) Int Dent J, 45 (1), pp. 27-34Oliveira, A.E., Almeida, S.M., Paganini, G.A., Haiter Neto, F., Bóscolo, F.N., Comparative study of two digjtal radiographic storage phosphor systems (2000) Braz Dent J, 11 (2), pp. 111-116Borg, E., Attaelmanan, A., Gröndahl, H.G., Image plate systems differ in physical performance (2000) Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 89 (1), pp. 118-124Martins, M.G.B.Q., Haiter Neto, F., Whaites, E.J., Analysis of digital images acquired using different phosphor storage plates (PSPs) subjected to varying reading times and storage conditions (2003) Dentomaxillofac Radiol, 32 (3), pp. 186-190(1998) DenOptix digital imaging system - user manual and installation guide, , Gendex Dental X-Ray Division, Des Plaines: The Division;Menig, J.J., The DenOptix digital radiographic system (1999) J Clin Orthod, 33 (7), pp. 407-410Berkhout WE, Beuger DA, Sanderink GC, van der Stelt PF. The dynamic range of digital radiographic systems: dose reduction or risk of overexposure? Dentomaxillofac Radiol. 2004;33(1):1-SHildebolt, C.F., Couture, R.A., Whiting, B.R., Dental photostimulable phosphor radiography (2000) Dent Clin North Am, 44 (2), pp. 273-297Martins, M.G.B.Q., Whaites, E.J., Ambrosano, G.M.B., Haiter Neto, F., What happens if you delay scanning Digora phosphor storage plates (PSPs) for up to 4 hours? (2006) Dentomaxillofac Radiol, 35 (3), pp. 143-146Gröndahl, H.G., Wenzel, A., Borg, E., Tammisalo, E., An image plate system for digital intra-oral radiography (1996) Dent Update, 23 (8), pp. 334-33

Electrooxidation and determination of dopamine using a Nafion®-cobalt hexacyanoferrate film modified electrode

The electrocatalysis of dopamine has been studied using a cobalt hexacyanoferrate film (CoHCFe)-modified glassy carbon electrode. Using a rotating disk CoHCFe-modified electrode, the reaction rate constant for dopamine was found to be 3.5 × 105 cm3 mol-1 s-1 at a concentration of 5.0 × 10-5 mol L-1. When a Nafion® film is applied to the CoHCFe-modified electrode surface a high selectivity for the determination of dopamine over ascorbic acid was obtained. The analytical curve for dopamine presented linear dependence over the concentration range from 1.2 × 10-5 to 5.0 × 10-4 mol L-1 with a slope of 23.5 mA mol-1 L and a linear correlation coefficient of 0.999. The detection limit of this method was 8.9 × 10-6 mol L-1 and the relative standard deviation for five measurements of 2.5 × 10-4 mol L-1 dopamine was 0.58%

Transforming growth factor-β (TGF-β) maintains follicular ultrastructure and stimulates preantral follicle growth in caprine ovarian tissue cultured in vitro

O objetivo desse estudo foi investigar se o TGF-β afeta a sobrevivência, ativação e crescimento de folículos primordiais caprinos inclusos no córtex ovariano após o cultivo in vitro. Ovários de cabras foram coletados em abatedouro e fragmentos de tecido ovariano foram cultivados por um e sete dias em meio essencial mínimo alfa (α-MEM+) sozinho ou suplementado com TGF-β (1, 5, 10 ou 50ng/mL). Fragmentos ovarianos não cultivados e cultivados foram processados para análise histológica e ultraestrutural. Os resultados mostraram que, comparado ao controle fresco, houve diminuição no percentual de folículos morfologicamente normais em todos os tratamentos somente após sete dias de cultivo. O TGF-β não afetou a ativação folicular independente da concentração testada, contudo, o diâmetro folicular foi superior (P<0.05) no tratamento com 10ng/mL de TGF-β quando comparado ao controle fresco e aos demais tratamentos. Além disso, essa mesma concentração manteve a ultraestrutura normal dos folículos após sete dias de cultivo. Em conclusão, o TGF-β apresentou efeito adicional no crescimento folicular e na manutenção da integridade ultraestrutural de folículos pré-antrais caprinos inclusos no tecido ovariano quando utilizado na concentração de 10ng/mL durante sete dias de cultivo.The objectives of this study were to investigate whether TGF-β affect the survival, activation and further growth of goat primordial follicles enclosed in ovarian cortex after in vitro culture. Goat ovaries were collected from an abattoir and pieces of ovarian tissues were cultured for one or seven days in a supplemented alpha Minimum Essential Medium, alone or containing TGF-β (1, 5, 10 or 50ng/mL). Ovarian tissues from the fresh control as well as those cultured were processed for histological and ultrastructural studies. The results showed that when compared with fresh control, there was decrease in the percentages of histologically normal follicles in all treatments only after seven days culture. TGF-β did not affect the activation of preantral follicles regardless of its concentration, however, larger follicles diameter (P<0.05) was observed using 10ng/mL TGF-β than in the fresh control and other treatments. Moreover, this concentration maintained the normal ultrastructure after seven days of culture. In conclusion, TGF-β showed additional effect on the follicle growth and the maintenance of ultrastructural integrity of goat preantral follicles enclosed in ovarian tissue when used at 10ng/mL during seven days of culture

Study of ATLAS sensitivity to FCNC top decays

The ATLAS experiment sensitivity to top quark Flavour Changing Neutral Current (FCNC) decays was studied at LHC using ttbar events. While one of the top quarks is expected to follow the dominant Standard Model decay t->bW, the other decays through a FCNC channel, i.e. t-> Z u(c), t-> gamma u(c) or t-> g u(c). Different types of analyses, applied to each FCNC decay mode, were compared. The FCNC branching ratio sensitivity (assuming a 5sigma signal significance) and 95% confidence level limits on the branching ratios (in the hypothesis of signal absence) were obtained

Genomic Variation and Arsenic Tolerance Emerged as Niche Specific Adaptations by Different Exiguobacterium Strains Isolated From the Extreme Salar de Huasco Environment in Chilean – Altiplano

Indexación: Scopus.Polyextremophilic bacteria can thrive in environments with multiple stressors such as the Salar de Huasco (SH). Microbial communities in SH are exposed to low atmospheric pressure, high UV radiation, wide temperature ranges, salinity gradient and the presence of toxic compounds such as arsenic (As). In this work we focus on arsenic stress as one of the main adverse factors in SH and bacteria that belong to the Exiguobacterium genus due to their plasticity and ubiquity. Therefore, our aim was to shed light on the effect of niche conditions pressure (particularly arsenic), on the adaptation and divergence (at genotypic and phenotypic levels) of Exiguobacterium strains from five different SH sites. Also, to capture greater diversity in this genus, we use as outgroup five As(III) sensitive strains isolated from Easter Island (Chile) and The Great Salt Lake (United States). For this, samples were obtained from five different SH sites under an arsenic gradient (9 to 321 mg/kg: sediment) and isolated and sequenced the genomes of 14 Exiguobacterium strains, which had different arsenic tolerance levels. Then, we used comparative genomic analysis to assess the genomic divergence of these strains and their association with phenotypic differences such as arsenic tolerance levels and the ability to resist poly-stress. Phylogenetic analysis showed that SH strains share a common ancestor. Consequently, populations were separated and structured in different SH microenvironments, giving rise to multiple coexisting lineages. Hence, this genotypic variability is also evidenced by the COG (Clusters of Orthologous Groups) composition and the size of their accessory genomes. Interestingly, these observations correlate with physiological traits such as growth patterns, gene expression, and enzyme activity related to arsenic response and/or tolerance. Therefore, Exiguobacterium strains from SH are adapted to physiologically overcome the contrasting environmental conditions, like the arsenic present in their habitat. © Copyright © 2020 Castro-Severyn, Pardo-Esté, Mendez, Morales, Marquez, Molina, Remonsellez, Castro-Nallar and Saavedra.https://www.frontiersin.org/articles/10.3389/fmicb.2020.01632/ful

On the critical behavior of disordered quantum magnets: The relevance of rare regions

The effects of quenched disorder on the critical properties of itinerant quantum antiferromagnets and ferromagnets are considered. Particular attention is paid to locally ordered spatial regions that are formed in the presence of quenched disorder even when the bulk system is still in the paramagnetic phase. These rare regions or local moments are reflected in the existence of spatially inhomogeneous saddle points of the Landau-Ginzburg-Wilson functional. We derive an effective theory that takes into account small fluctuations around all of these saddle points. The resulting free energy functional contains a new term in addition to those obtained within the conventional perturbative approach, and it comprises what would be considered non-perturbative effects within the latter. A renormalization group analysis shows that in the case of antiferromagnets, the previously found critical fixed point is unstable with respect to this new term, and that no stable critical fixed point exists at one-loop order. This is contrasted with the case of itinerant ferromagnets, where we find that the previously found critical behavior is unaffected by the rare regions due to an effective long-ranged interaction between the order parameter fluctuations.Comment: 16 pp., REVTeX, epsf, 2 figs, final version as publishe